Assessment of Antioxidant, Anti inflammatory and Anti microbial efficacy of Piper longum and Piper betle mediated titanium dioxide nanoparticles


  • *Dr. Vamshi Nizampuram, Dr. Sahana Selvaganesh, MS, Dr. Rajeshkumar S, Dr. Thiyaneswaran Nesappan


Titanium-nanoparticles, Green-synthesis, Piper longum, Piper betle, Antibacterial, Anti Inflammatory, Anti Oxidant.


Background: Titanium nanoparticles (TiO2 NPs) hold promise for various applications due to their unique properties. This study presents a novel green synthesis method utilizing Piper longum and Piper betle extracts for the first time. The investigation further explores the potential antimicrobial, antioxidant, and anti-inflammatory activities of the synthesized nanoparticles.

Methods: The synthesized TiO2 NPs were characterized using various techniques (UV-Vis, FT-IR, SEM, XRD) to confirm their formation and properties. Subsequently, their antibacterial efficacy against common pathogens (Streptococcus mutans, E. faecalis, Staphylococcus aureus, Candida albicans) was evaluated. The nanoparticles were also assessed for their antioxidant activity using the H2O2 assay and anti-inflammatory potential through the egg albumin denaturation assay.

Results: The synthesized nanoparticles exhibited a zone of inhibition of 9 ± 0.5 mm against bacterial strains, comparable to established standards. Notably, they demonstrated a maximum anti-inflammatory inhibition of 80.83% and displayed promising antioxidant activity with a maximum inhibition of 86.23% at a concentration of 50 μg/ml.

Conclusion: This study successfully established a green synthesis approach for TiO2 NPs using P. longum and P. betle. The resulting nanoparticles demonstrated remarkable antibacterial, antioxidant, and anti-inflammatory properties, suggesting their potential for future applications in therapeutic interventions and immunomodulation.

Categories: Nano biotechnology, Health care.