Comparing Pre And Post Exposure Genotoxic Effects Of Radiation On Buccal Epithelial Cells Using Exfoliative Cytology- An Observational Study
Keywords:
Cytologic alterations, Spiral Cone Beam Computed Tomography, Pantomography, Mn, oral mucosal cells. Key message:Considering genomic alterations caused by radiation as shown in this study we recommend judiciary use of 3D radiographs whenever necessary.Abstract
Ionizing radiations causes damage to DNA and DNA protein crosslinks and induces cellular death. Use of ionizing radiation is unavoidable in certain dental disorders thus leading to stochastic effects on exposed tissue. Present study is undertaken to evaluate association of routine diagnostics radiographic examination to increased risk of cancer due to cytotoxic effect of x-ray imaging and to minimize potential risk by prescribing radiographs where deemed necessary and using appropriate modality required.
Aim: To evaluate post exposure genotoxic effects of radiation on buccal epithelial cells using exfoliative cytology.
Objectives:To detect number of Micronuclei(Mn) in exfoliated buccal cells of adult male and female patients following radiographic examination using CBCT of single volume (SV), CBCT of single arch (SA) and a orthopantomogram (OPG).
Material and methods:Eighty seven healthy patients were recruited who fulfilled the inclusion and exclusion criteria. Patients were divided into three groupsExfoliated buccal cells were collected before CBCT and OPG exposure, thereafter 7-10 days post exposure and 18-20 days after exposure. Cytological smears were examined to detect changes in number of Mn.
Conclusion:Present study concludes that, radiation exposure from CBCT as well as OPG has inevitable health side effects that should be taken into consideration while prescribing diagnostic radiographs.
